target enrichment of UCEs and exons from anthozoans - SRA

SRX7138444: target enrichment of UCEs and exons from anthozoans
1 ILLUMINA (Illumina HiSeq 3000) run: 2.7M spots, 820.2M bases, 377Mb downloads

Design: We prepared DNA libraries using the Kapa Biosystems Hyper Prep Kit with dual indexed iTru adapters (Glenn et al. 2016). The resulting DNA libraries were combined in equimolar pools of 5 to 8 samples. Pooled libraries were enriched for UCE loci using the anthozoa-v1 bait set (Quattrini et al. 2017) synthesized by MYcroarray (Ann Arbor, MI). We quantified each enriched pool, post- PCR amplification, using a Qubit fluorometer and checked them for adapter contamination using a Bioanalyzer. If adapters were found, a 1X bead cleanup was performed. After cleaning up any remaining adapter contamination, we qPCR quantified each pooled library using the Kapa qPCR quantification kit, and we combined pooled libraries prior to sequencing.

Submitted by: Harvey Mudd College

Study: Phylogenomics of Anthozoa: New Approaches to long-standing problems

PRJNA588468 • SRP229811 • All experiments • All runs

show Abstracthide Abstract

Sequence data were captured using a targeted enrichment approach for UCEs and exons.

Sample:

SAMN13245005 • SRS5647113 • All experiments • All runs

Organism: Bebryce sp. CSM-2012

Library:

Name: ANT54

Instrument: Illumina HiSeq 3000

Strategy: WGS